Abstract - Carlos Portera-Cailliau

The ability to resolve the fast fluorescent transients associated with neuronal action potential firing over a large volume of brain tissue will enable new directions in modern neuroscience research, leading to a better understanding of neural circuit connectivity and activity. The goal of this proposal is to develop a highly innovative fluorescence microscopy instrumentation technology for high-speed neural imaging applications, capable of kilohertz frame rates with sufficient sensitivity and resolution to resolve the millisecond-timescale dynamics of neuronal ensemble activity in the awake brain of mice. We intend to leverage our combined expertise in developing cutting edge techniques in radiofrequency communications (Jalali lab) and in in vivo two-photon calcium imaging of network activity in mice (Portera-Cailliau lab). The new approach will combine Multi-photon excitation and a technique we recently developed called Fluorescence Imaging using Radiofrequency-tagged Emission (mFIRE). We will benchmark the performance of mFIRE against the conventional state-of-the-art two-photon calcium imaging to demonstrate its unprecedented time resolution for calcium imaging of neuronal ensemble activity in the neocortex using genetically encoded fluorescent calcium indicators and voltage sensors. We anticipate that mFIRE will greatly facilitate new discoveries in all areas of neuroscience, which is a primary goal of the Cal-Brain initiative.

» Back to Awards


Principal Investigator Institution Title Abstract
Andersen, Richard California Institute of Technology Engineering Artificial Sensation View
Andrews, Anne University of California, Los Angeles Nanoscale Neurotransmitter Sensors View
Bloodgood, Brenda University of California San Diego A novel toolkit for visualizing and manipulating activity-induced transcription in living brain. View
Chaumeil, Myriam University of California, San Francisco In vivo metabolic imaging of neuroinflammation using hyperpolarized 13C View
Cleary, Michael University of California, Merced Capturing physiological maps of neural gene expression View
Cohen, Bruce University of California, Lawrence Berkeley National Laboratory Nano-optogenetic control of neuronal firing with targeted nanocrystals View
Dai, Hongjie Stanford University  Deep brain imaging of single neurons in the second near-infrared optical window View
Hall, Drew University of California, San Diego Magnetic Monitoring of Neural Activity using Magnetoresistive Nanosensors View
Krubitzer, Leah University of California, Davis An integrated system to monitor, image, and regulate neural activity View
Kubby, Joel University of California, Santa Cruz Three-Photon Microscopy with Adaptive Optics for Deep Tissue Brain Activity Imaging View
Melosh, Nicholas Stanford University Parallel Solid State Intracellular Patch-Clamping with Biomimetic Probes View
Park, B. Hyle University of California, Riverside  Label-free 4D optical detection of neural activity View
Portera-Cailliau, Carlos University of California, Los Angeles High-speed interrogation of network activity with frequency domain multiplexing View
Shanechi, Maryam University of Southern California Control-Theoretic Neuroprosthetic Design Using Electrocorticography Signals View
Smith, Will University of California, Santa Barbara Whole brain imaging in a primative chordate View
Wood, Marcelo University of California, Irvine Epigenetic PET tracer for cross-species investigation of age-related memory dysfunction View