Abstract - Carlos Portera-Cailliau
The ability to resolve the fast fluorescent transients associated with neuronal action potential firing over a large volume of brain tissue will enable new directions in modern neuroscience research, leading to a better understanding of neural circuit connectivity and activity. The goal of this proposal is to develop a highly innovative fluorescence microscopy instrumentation technology for high-speed neural imaging applications, capable of kilohertz frame rates with sufficient sensitivity and resolution to resolve the millisecond-timescale dynamics of neuronal ensemble activity in the awake brain of mice. We intend to leverage our combined expertise in developing cutting edge techniques in radiofrequency communications (Jalali lab) and in in vivo two-photon calcium imaging of network activity in mice (Portera-Cailliau lab). The new approach will combine Multi-photon excitation and a technique we recently developed called Fluorescence Imaging using Radiofrequency-tagged Emission (mFIRE). We will benchmark the performance of mFIRE against the conventional state-of-the-art two-photon calcium imaging to demonstrate its unprecedented time resolution for calcium imaging of neuronal ensemble activity in the neocortex using genetically encoded fluorescent calcium indicators and voltage sensors. We anticipate that mFIRE will greatly facilitate new discoveries in all areas of neuroscience, which is a primary goal of the Cal-Brain initiative.
AWARDS
| Principal Investigator | Institution | Title | Abstract |
| Andersen, Richard | California Institute of Technology | Engineering Artificial Sensation | View |
| Andrews, Anne | University of California, Los Angeles | Nanoscale Neurotransmitter Sensors | View |
| Bloodgood, Brenda | University of California San Diego | A novel toolkit for visualizing and manipulating activity-induced transcription in living brain. | View |
| Chaumeil, Myriam | University of California, San Francisco | In vivo metabolic imaging of neuroinflammation using hyperpolarized 13C | View |
| Cleary, Michael | University of California, Merced | Capturing physiological maps of neural gene expression | View |
| Cohen, Bruce | University of California, Lawrence Berkeley National Laboratory | Nano-optogenetic control of neuronal firing with targeted nanocrystals | View |
| Dai, Hongjie | Stanford University | Deep brain imaging of single neurons in the second near-infrared optical window | View |
| Hall, Drew | University of California, San Diego | Magnetic Monitoring of Neural Activity using Magnetoresistive Nanosensors | View |
| Krubitzer, Leah | University of California, Davis | An integrated system to monitor, image, and regulate neural activity | View |
| Kubby, Joel | University of California, Santa Cruz | Three-Photon Microscopy with Adaptive Optics for Deep Tissue Brain Activity Imaging | View |
| Melosh, Nicholas | Stanford University | Parallel Solid State Intracellular Patch-Clamping with Biomimetic Probes | View |
| Park, B. Hyle | University of California, Riverside | Label-free 4D optical detection of neural activity | View |
| Portera-Cailliau, Carlos | University of California, Los Angeles | High-speed interrogation of network activity with frequency domain multiplexing | View |
| Shanechi, Maryam | University of Southern California | Control-Theoretic Neuroprosthetic Design Using Electrocorticography Signals | View |
| Smith, Will | University of California, Santa Barbara | Whole brain imaging in a primative chordate | View |
| Wood, Marcelo | University of California, Irvine | Epigenetic PET tracer for cross-species investigation of age-related memory dysfunction | View |