Abstract - Hongjie Dai

High spatial and temporal resolution brain imaging at the single neuron level in real-time could provide valuable information of cellular structures, behaviors and processes for understanding brain function and related diseases in unprecedented details. Thus far brain imaging techniques are limited in spatial resolution and time resolution. Fluorescence imaging in the visible of near infrared (NIR) of ~800 nm are limited to shallow tissue imaging depth of ~ 0.2 mm due to strong photon scattering and require invasive scalp and skull removal through cranial windows. Here we will innovate NIR-II (1000-1700nm) fluorescence imaging methodologies capable of non-invasive through skull imaging at millimeters depth into the brain. Negligible tissue autofluorescence and reduced photon scattering in the long wavelength NIR-II window up to ~ 1700nm will allow for single-cell resolution at up to millimeters-scale depth in vivo. Specific aims will include, (1) Synthesis of NIR-II fluorophores with high quantum yield and tunable fluorescence emission up to 1700nm including Ca2+ indicator dyes emitting in the NIR-II window. (2) Perform through skull in vivo imaging of single neurons (with sub-10 micron resolution) in mouse brain at millimeters depth to glean single neuron structures and responses and activities at unprecedented depths.

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AWARDS

Principal Investigator Institution Title Abstract
Andersen, Richard California Institute of Technology Engineering Artificial Sensation View
Andrews, Anne University of California, Los Angeles Nanoscale Neurotransmitter Sensors View
Bloodgood, Brenda University of California San Diego A novel toolkit for visualizing and manipulating activity-induced transcription in living brain. View
Chaumeil, Myriam University of California, San Francisco In vivo metabolic imaging of neuroinflammation using hyperpolarized 13C View
Cleary, Michael University of California, Merced Capturing physiological maps of neural gene expression View
Cohen, Bruce University of California, Lawrence Berkeley National Laboratory Nano-optogenetic control of neuronal firing with targeted nanocrystals View
Dai, Hongjie Stanford University  Deep brain imaging of single neurons in the second near-infrared optical window View
Hall, Drew University of California, San Diego Magnetic Monitoring of Neural Activity using Magnetoresistive Nanosensors View
Krubitzer, Leah University of California, Davis An integrated system to monitor, image, and regulate neural activity View
Kubby, Joel University of California, Santa Cruz Three-Photon Microscopy with Adaptive Optics for Deep Tissue Brain Activity Imaging View
Melosh, Nicholas Stanford University Parallel Solid State Intracellular Patch-Clamping with Biomimetic Probes View
Park, B. Hyle University of California, Riverside  Label-free 4D optical detection of neural activity View
Portera-Cailliau, Carlos University of California, Los Angeles High-speed interrogation of network activity with frequency domain multiplexing View
Shanechi, Maryam University of Southern California Control-Theoretic Neuroprosthetic Design Using Electrocorticography Signals View
Smith, Will University of California, Santa Barbara Whole brain imaging in a primative chordate View
Wood, Marcelo University of California, Irvine Epigenetic PET tracer for cross-species investigation of age-related memory dysfunction View